|Every 42 seconds, someone in the US is arrested for marijuana use.||
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Study Confirms Cannabis Oil Cures Cancer.
2002 - News ~ Lymphoma may be slowed by cannabisThe Peripheral Cannabinoid Receptor CB2 and CD40 Are Novel Biological Markers That Predict Outcome in Diffuse Large B-Cell Lymphoma of Elderly Patients. Cannabinoid Receptor-Mediated Apoptosis Induced by R(+)-Methanandamide and Win55,212-2 Is Associated with Ceramide Accumulation and p38 Activation in Mantle Cell Lymphoma Potentiation of cannabinoid-induced cytotoxicity in mantle cell lymphoma through modulation of ceramide metabolism.
2009 - News ~ Medical Marijuana and Lymphoma
Expression of cannabinoid receptors type 1 and type 2 in non-Hodgkin lymphoma: growth inhibition by receptor activation
Int J Cancer. 2008 Sep 1;123(5):1025-33.
Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet and Karolinska University Hospital Huddinge, F-46, SE-14186 Stockholm, Sweden.
Endogenous and synthetic cannabinoids exert antiproliferative and proapoptotic effects in various types of cancer and in mantle cell lymphoma (MCL). In this study, we evaluated the expression of cannabinoid receptors type 1 and type 2 (CB1 and CB2) in non-Hodgkin lymphomas of B cell type (n = 62).
A majority of the lymphomas expressed higher mRNA levels of CB1 and/or CB2 as compared to reactive lymphoid tissue. With the exception of MCL, which uniformly overexpresses both CB1 and CB2, the levels of cannabinoid receptors within other lymphoma entities were highly variable, ranging from 0.1 to 224 times the expression in reactive lymph nodes. Low levels of the splice variant CB1a, previously shown to have a different affinity for cannabinoids than CB1, were detected in 44% of the lymphomas, while CB1b expression was not detected.
In functional studies using MCL, Burkitt lymphoma (BL), chronic lymphatic leukemia (CLL) and plasma cell leukemia cell lines, the stable anandamide analog R(+)-methanandamide (R(+)-MA) induced cell death only in MCL and CLL cells, which overexpressed both cannabinoid receptors, but not in BL. In vivo treatment with R(+)-MA caused a significant reduction of tumor size and mitotic index in mice xenografted with human MCL.
Together, our results suggest that therapies using cannabinoid receptor ligands will have efficiency in reducing tumor burden in malignant lymphoma overexpressing CB1 and CB2.
Cannabinoid Receptor Agonists May Be Novel Class of Anti-Lymphoma Agents
Publication date: July 25, 2002
Author: Faith Reidenbach
NEW YORK (Reuters Health) Jul 25 - Delta-9-tetrahydrocannibinol (THC), the major
component of marijuana, and other cannabinoids induce apoptosis in murine tumors of
immune origin, according to researchers at Virginia Commonwealth University in Richmond.
Like other immune cells, cancers of the immune system express a cannabinoid receptor
known as CB2, Dr. Mitzi Nagarkatti explained in an interview with Reuters Health.
Compounds that bind CB2 receptors selectively induce apoptosis in these cancer cells, she
said. Moreover, "compounds that interact with CB2 will not exhibit psychotropic effects."
In a series of in vitro experiments, Dr. Nagarkatti and her colleagues exposed murine
lymphoma and mastocytoma cells to four cannabinoid receptor agonists. THC and two of the
others significantly reduced cell viability and increased apoptosis, they report in the July
15th issue of Blood.
In vivo experiments confirmed the effect of THC. Ten days after mice were injected with
lymphoma cells, cells collected from animals treated with the highest dose of THC showed
77.3% apoptosis. Two weeks of THC treatment cured 25% of lymphoma-bearing mice.
"It is possible that the immunosuppressive effects of THC may have interfered with the
host's antitumor immunity, which may account for a lower percentage of cures," the
researchers comment. They are currently conducting murine dose-ranging studies.
The research group also demonstrated that three human leukemia and lymphoma cell lines
expressed CB2 and not CB1. Three cannabinoids, including THC, induced apoptosis in these
cell lines in vitro, and THC showed the same effect when cultured with cells from patients
diagnosed with acute lymphoblastic leukemia.
"Recently, however, we identified a human cell line that was resistant," Dr. Nagarkatti's
team reports. "Further studies are in progress to address whether this cell line lacks physical
or functional cannabinoid receptors and/or signaling molecules that trigger apoptosis."
In addition, the research team is currently "screening a large number of CB2 analogs to
identify compounds that are highly efficacious in killing the cancer cells," Dr. Nagarkatti said.
"We are also investigating whether endogenous cannabinoids can exert antitumor activity."
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